Physicochemical and microbiological characterization of cement kiln dust for potential reuse in wastewater treatment.

Cement kiln dust (CKD), a byproduct of cement manufacturing process, was collected from Misr Cement Co. at Qena, Egypt. CKD was characterized by X-ray diffraction and FTIR analysis. This byproduct was investigated for its physical-chemical characters, antibacterial activities on sewage water and the presence of nematode, parasites and algae in the treated water. The efficiency of CKD-treated water was also examined on Hibiscus sabdarriffa seed germination. Total bacteria, total and fecal coliform, as well as fecal streptococci were completely inhibited by CKD. Interestingly, zinc, manganese, iron, nickel and lead were completely absent from sewage water as these metals precipitated after treatment with 10gl(-1) CKD. On the other hand, among all the tested plant criteria, only root length was significantly reduced by 55% and 15% after zero and 3 days of CKD addition respectively compared to control. Furthermore, plant lipid peroxidation showed no significant differences between treated sewage water and control after zero and 3 days time addition of CKD. Catalase enzyme activity showed significant decrease by 56% and 64%, while peroxidase activity significantly increased up to 49% and 63% compared to untreated sewage after zero and 3 days of treatment, respectively. The absorption of lead, iron and copper by treated and untreated plants showed no significant differences. Chromium ions were highly absorbed (0.075mgl(-1)) by plants irrigated only with treated sewage water at zero time, and decreased gradually to 0.018mgl(-1) after 3 days of CKD addition. This study highlighted the efficiency of cement kiln dust as an antibacterial agent and its ability of scavenging heavy metals leading to the use of treated sewage water in activities such as crop irrigation.

Preliminary evidence on photoreactivation of Frankia spores with visible light after exposure to UV-C radiation.

Spores of four Frankia strains, the nitrogen-fixing actinomycete, were exposed to short wavelength UV-C radiation of 254 nm at 1 lux cm(2) (0.24 mw cm2 of energy) for 10 min. The used strains were HFP020203, UGL020604, UGL020602q and ORS021001. Exposure to UV was followed by reactivation with visible white light at 327.4 lux cm(2) for the same period of time. Spore germination percentage, spore protein content, and cell growth were damaged by this treatment. The lower and higher percentages of reduction in spore germination were 32 and 63% and, for the same strains, the recovery by white light was 7.2 and 37%. The lower percentages of UV damage and subsequent low recovery were recorded for strain ORS021001 that is considered more resistant to UV than the other strains. The higher percentages were recorded for strain HFP020203 that is more sensitive to UV but having more efficient repairing mechanisms. All the tested strains showed repairing activity induced by white light as indicated from the increase in their spore germination, protein content and almost restoring the normal shape of Frankia hyphae, after being damaged, as revealed by scanning electron microscope. This is the first evidence that photo-repairing systems are present in Frankia strains although there are variations in their response to both UV-C and photoreactivation by white light.

Improving Casuarina growth and symbiosis with Frankia under different soil and environmental conditions--review.

Casuarinas are very important plants for their various uses and survival in adverse sites or harsh environments. As nitrogen fixation, in symbiosis with Frankia, is an important factor for the survival of these plants under various conditions, the basis for selecting both effective and tolerant Frankia strains and Casuarina spp., are provided. Enhancement of the symbiotic relationship between Frankia and Casuarina, by mycorrhizal infection and other biofertilizing microorganisms such as Bacillus and Azospirillum, is reflected by superior plant growth. Casuarina leaf litter is also a great source for both inorganic and organic nutrients. Therefore, careful management of the top soil layer under Casuarina trees is very important. Litter decomposition ratio is affected by many physical chemical and biological factors including temperature, moisture conditions, lignin, and C-to-N and N-to-P ratios in addition to soil biota. In general, here the above relations are discussed and an alleviation model is presented for important disturbances of natural and human origin made in soil and environment, especially in the dry regions. In conclusion, we suggest how to optimize the nitrogen fixation and plant growth under the prevalent conditions.

Effects of storage time and temperature on the infectivity and effectiveness of Frankia entrapped in polyacrylamide gel.

Four Frankia-Casuarina endosymbiont strains were tested for their infectivity and effectiveness on C. equisetifolia plants after 1 d, 3 and 6 months of Frankia storage at 7, 28 and 40 degrees C as liquid-cultures and polyacrylamide gel (PAG)-immobilized inocula. At lower temperature the number of nodules was the same or higher than control for liquid inocula except after 6 months of storage. For the PAG-entrapped Frankia lower numbers of nodules were recorded due to the use of high Frankia titers. In general, the results showed comparable plant dry mass, total nitrogen, root to shoot and nodules to plant ratios at lower temperature treatments. Increasing time and temperature was accompanied with reduced infectivity and effectiveness on inoculated plants. No nodulation was scored on plants inoculated with liquid and PAG-entrapped inocula stored at 40 degrees C for 6 months; subsequently, plant growth was inhibited. The variations in results obtained for different strains and treatments lead to variations in plant nitrogen-fixing ability. The use of PAG as a carrier for different Frankia strains is suggested; the recommended storage temperature for PAG-immobilized Frankia in 7-28 degrees C for up to 3 months.

Composition of Casuarina leaf litter and its influence on Frankia-Casuarina symbiosis in soil.

Plant needles of Casuarina equisetifolia were collected and analyzed in parallel with soil analysis. In three strains of Frankia--symbionts of Casuarina--their infectivity and plant performance was determined in vitro after soil amendment with different leaf litter concentrations. Only one strain was able to nodulate the plant at all litter concentrations (0.5, 3 and 5%) although the nodules were very small. However, all treated plants grew poorly; their growth was reduced by approximately 90% (for 5% litter concentration) compared to plants grown on untreated soil, on the basis of total dry mass. Inhibition of nodulation can be attributed to high concentrations of some elements and compounds that were either found in C. equisetifolia litter or originally found in soil (i.e. chloride, cyanide, copper, manganese and phenols). In general, plant growth decreased as more litter was added. Plant total nitrogen content was also reduced after increasing the litter concentration. The inhibitory effect of high litter concentrations was mainly on plant growth and to a lesser extent on plant nodulation by Frankia strains.

Effect of Al, Co, and Pb ions on growth of Frankia spp. in a mineral medium.

Growth of three Frankia strains associated with roots of Casuarina tree, treated with different concentrations of metal ions, was measured as total protein content. One strain was highly resistant to different aluminum ion concentrations up to 10 mmol/L. The other two strains were more sensitive to the higher aluminum concentrations (1.25-10 mmol/L). Growth inhibition by cobalt and lead concentrations varied, depending on the tested strain. Stimulation occurred only at cobalt concentrations of 0.33 and 0.65 mmol/L for one strain.